4L6A
Structure of human mitochondrial 5'(3')-deoxyribonucleotidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-02 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.255 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.410, 73.410, 106.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.498 - 1.400 |
R-factor | 0.141 |
Rwork | 0.141 |
R-free | 0.15400 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.017 |
RMSD bond angle | 2.087 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.498 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.472 | |
Number of reflections | 57600 | |
<I/σ(I)> | 11.9 | 1.6 |
Redundancy | 5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 8% PEG 8000, 20MM POTASSIUM PHOSPHATE, 10% GLYCEROL, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |