4L5J
Crystal structures of the LsrR proteins complexed with phospho-AI-2 and its two different analogs reveal distinct mechanisms for ligand recognition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 189.330, 80.611, 118.701 |
Unit cell angles | 90.00, 118.01, 90.00 |
Refinement procedure
Resolution | 47.320 - 2.600 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.320 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 45072 | |
Completeness [%] | 78.4 | 92.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1 M Bis-Tris, 2% isopropanol, 8% gamma-butyrolactone, pH 6.5, VAPOR DIFFUSION, temperature 293K |