4L59
Crystal structure of the 3-MBT repeat domain of L3MBTL3 and UNC2533 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 104.798, 104.798, 110.644 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.400 - 2.290 |
| R-factor | 0.2128 |
| Rwork | 0.212 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fl6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.290 | 2.300 |
| Rmerge | 0.073 | 0.872 |
| Number of reflections | 31431 | |
| <I/σ(I)> | 33.9 | |
| Completeness [%] | 98.9 | 99.7 |
| Redundancy | 8.6 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 1.5M Na Citrate, 0.1M HEPES pH7.5, temperature 291K, VAPOR DIFFUSION, HANGING DROP |
