4L4T
Structure of human MAIT TCR in complex with human MR1-6-FP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 217.228, 69.555, 142.892 |
| Unit cell angles | 90.00, 104.42, 90.00 |
Refinement procedure
| Resolution | 49.380 - 2.000 |
| R-factor | 0.1865 |
| Rwork | 0.184 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gup |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.121 | 0.747 |
| Number of reflections | 139624 | |
| <I/σ(I)> | 10.5 | 2.1 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
