4L47

Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	210.200, 450.230, 621.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.966 - 3.220
R-factor	0.23075614842
Rwork	0.229
R-free	0.25983
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3I9D AND 3I9E
RMSD bond length	0.006
RMSD bond angle	1.101
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.966		3.260
High resolution limit [Å]	3.180	14.220	3.180
Rmerge	0.249	0.016	0.016
Number of reflections	962803	10555	70620
<I/σ(I)>	7.88	38.28	1.08
Completeness [%]	98.5	90.5	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K