4L44
Crystal structures of human p70S6K1-T389A (form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-26 |
| Detector | CCD ADSC unsupported-q315 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 77.961, 77.961, 113.829 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.2459 |
| Rwork | 0.243 |
| R-free | 0.29780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.576 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.134 | 0.093 | 0.376 |
| Number of reflections | 9546 | ||
| <I/σ(I)> | 9.7 | ||
| Completeness [%] | 98.8 | 97.5 | 99.7 |
| Redundancy | 3.9 | 3.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.2 | 289 | 2M lithium sulfate, 0.05M magnesium sulfate, 0.05M HEPES, pH 7.2, hanging drop, temperature 289K |
