4L42
Crystal structures of human p70S6K1-PIF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-09 |
| Detector | CCD ADSC unsupported-q315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 67.656, 67.656, 377.735 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.2297 |
| Rwork | 0.228 |
| R-free | 0.26250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a62 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.529 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.114 | 0.079 | 0.496 |
| Number of reflections | 13601 | ||
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 99.3 | 95.6 | 99.9 |
| Redundancy | 3.4 | 3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289 | 2.0M ammonium sulfate, 0.1M MES, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
