4L35
Crystal structure of cruxrhodopsin-3 at pH5 from Haloarcula vallismortis at 2.1 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 106.010, 106.010, 55.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.2314 |
| Rwork | 0.231 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jr8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.152 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.460 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.079 | 0.494 |
| Number of reflections | 19657 | |
| <I/σ(I)> | 20.8 | 4.6 |
| Completeness [%] | 93.1 | 96.9 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 288 | 2.5M ammonium sulfate, 0.1M Na-citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
