4L2W
Crystal structure of the Shroom-Binding domain of human Rock1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-11-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.500, 56.230, 80.740 |
| Unit cell angles | 90.00, 119.14, 90.00 |
Refinement procedure
| Resolution | 38.315 - 2.490 |
| R-factor | 0.2379 |
| Rwork | 0.236 |
| R-free | 0.27560 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.838 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.490 | 6.780 | 2.490 |
| Rmerge | 0.076 | 0.044 | 0.547 |
| Number of reflections | 17172 | ||
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 88.6 | 95.8 | 56.1 |
| Redundancy | 5.7 | 5.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 277 | 0.1M Citrate, 1.0M Ammonium Sulfate, pH 6.0, VAPOR DIFFUSION, temperature 277K |
