4L1T
Transthyretin in complex with (E)-3-(dimethylamino)-5-(4-hydroxy-3,5-dimethylstyryl)benzoic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 200 |
Detector technology | PIXEL |
Collection date | 2012-08-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9797 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 42.305, 85.551, 63.006 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.920 - 1.160 |
R-factor | 0.1648 |
Rwork | 0.164 |
R-free | 0.18790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fbr |
RMSD bond length | 0.016 |
RMSD bond angle | 1.692 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.920 | 1.220 |
High resolution limit [Å] | 1.160 | 1.160 |
Number of reflections | 78960 | |
<I/σ(I)> | 20.3 | 2.3 |
Completeness [%] | 98.3 | 90.5 |
Redundancy | 6 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | The WT-TTR was concentrated to 4 mg/mL in 10 mM NaPi, 100 mM KCl, at pH 7.6 and co-crystallized at room temperature using the vapor diffusion sitting drop method. Crystals were grown from 1.395 M sodium citrate, 3.5% v/v glycerol at pH 5.5. The crystals were frozen using a cryo-protectant solution of 1.395 M sodium citrate, pH 5.5, containing 10% v/v glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |