4L00

Crystal structure of the apo Jak1 pseudokinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97720
Spacegroup name	P 1 21 1
Unit cell lengths	47.026, 80.656, 76.231
Unit cell angles	90.00, 95.44, 90.00

Refinement procedure

Resolution	28.890 - 1.800
R-factor	0.17052
Rwork	0.169
R-free	0.20302
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.569
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.800
Number of reflections	48311
Completeness [%]	97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP