4KY1
humanized HP1/2 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 82.010, 100.902, 140.188 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.660 - 2.970 |
| R-factor | 0.2097 |
| Rwork | 0.206 |
| R-free | 0.28430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fzu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.616 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 2.970 | 6.460 | 3.000 |
| Rmerge | 0.119 | 0.085 | 0.418 |
| Number of reflections | 12244 | ||
| <I/σ(I)> | 6.9 | ||
| Completeness [%] | 99.8 | 99.5 | 98.9 |
| Redundancy | 10.8 | 10 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 23% PEG 3350 and 0.2 M ammoniumsulfate, VAPOR DIFFUSION, HANGING DROP, temperature 297K, pH 7.5 |
