4KX6

Plasticity of the quinone-binding site of the complex II homolog quinol:fumarate reductase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	295.15
Detector technology	IMAGE PLATE
Collection date	2012-04-07
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	97.093, 138.620, 273.263
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.800 - 2.950
R-factor	0.24087
Rwork	0.240
R-free	0.27976
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.997
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.800	50.000	3.060
High resolution limit [Å]	2.950	6.350	2.950
Number of reflections	75158
Completeness [%]	95.7	93.7	94.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	295.15	13%-15% PEG 5000 MME, 85mM-115mM Magnesium Acetate, 100mM Sodium Citrate, 0.01mM DTT, 0.1mM EDTA, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K