4KV5
scFv GC1009 in complex with TGF-beta1.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-15 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.188, 171.769, 109.876 |
Unit cell angles | 90.00, 111.30, 90.00 |
Refinement procedure
Resolution | 30.960 - 3.000 |
R-factor | 0.2329 |
Rwork | 0.229 |
R-free | 0.30030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.384 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.960 | 30.960 | 3.160 |
High resolution limit [Å] | 3.000 | 6.550 | 3.050 |
Rmerge | 0.059 | 0.467 | |
Number of reflections | 36020 | ||
<I/σ(I)> | 14.1 | 1.9 | |
Completeness [%] | 99.8 | 98.9 | 99.8 |
Redundancy | 3.2 | 3.38 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 294 | 16% PEG 4K, 0.1 M citrate, 4% 2-propanol , pH 5.0, VAPOR DIFFUSION, temperature 294K |