4KU2
Crystal Structure C143A from Xanthomonas campestris Bound with Myristoyl-CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-02-12 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.03 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.204, 85.379, 102.706 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.776 - 1.974 |
R-factor | 0.1591 |
Rwork | 0.157 |
R-free | 0.19910 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3row |
RMSD bond length | 0.013 |
RMSD bond angle | 1.321 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.010 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.055 | 0.417 |
Number of reflections | 47113 | |
<I/σ(I)> | 29.7 | 4.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 18% PEG 8000, 80 mM potassium phosphate dibasic, 100 mM sodium citrate pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |