4KTS
Bovine trypsin in complex with microviridin J at pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.330, 64.490, 71.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.300 |
| R-factor | 0.10716 |
| Rwork | 0.106 |
| R-free | 0.12829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3myw |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.086 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.043 | 0.448 |
| Number of reflections | 65291 | |
| <I/σ(I)> | 20.6 | 4.6 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris, 0.2 M lithium sulfate, 25% PEG 3350 , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
