4KSI
Crystal Structure Analysis of the Acidic Leucine Aminopeptidase of Tomato
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-07-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 160.597, 160.597, 104.183 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.140 - 2.200 |
| R-factor | 0.13854 |
| Rwork | 0.137 |
| R-free | 0.17350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.749 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.136 | 0.628 |
| Number of reflections | 38529 | |
| <I/σ(I)> | 23.618 | 5.256 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.9 | 16.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M MES, 0.1 M ammonium sulfate, 45% (v/v) MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
