4KRR

Crystal structure of Drosophila WntD N-terminal domain-linker (residues 31-240)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-28
Detector	PSI PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 41
Unit cell lengths	59.625, 59.625, 66.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.812 - 2.124
R-factor	0.1805
Rwork	0.179
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f0a
RMSD bond length	0.002
RMSD bond angle	0.561
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.625	29.813	2.240
High resolution limit [Å]	2.124	6.720	2.124
Rmerge		0.016	0.016
Total number of observations		5592	26418
Number of reflections	13301
<I/σ(I)>	23.2	22.6	0.5
Completeness [%]	99.9	98.1	99.9
Redundancy	13.5	12.9	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	283	20% PEG 1000, 0.1M Bis-Tris, 0.2M magnesium chloride, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K