4KPS
Structure and receptor binding specificity of the hemagglutinin H13 from avian influenza A virus H13N6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.638, 76.804, 76.380 |
| Unit cell angles | 85.60, 82.67, 87.52 |
Refinement procedure
| Resolution | 42.455 - 2.587 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f23 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.896 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.587 | 2.600 |
| Number of reflections | 50525 | |
| Completeness [%] | 95.7 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.2M L-Proline, 0.1M HEPES, 10% PEG-3350, 0.01M Sodium Bromide, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
