4KP3
Crystal Structure of MyoVa-GTD in Complex with Two Cargos
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Collection date | 2012-12-15 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.850, 107.933, 83.510 |
Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
Resolution | 38.694 - 2.405 |
R-factor | 0.1945 |
Rwork | 0.192 |
R-free | 0.24110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wb8 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.769 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.067 |
Number of reflections | 54968 |
<I/σ(I)> | 24.7 |
Completeness [%] | 98.4 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 12-16%(w/v) PEG3350, 2-4% tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |