4KP1
Crystal structure of IPM isomerase large subunit from methanococcus jannaschii (MJ0499)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.457, 98.838, 142.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.036 - 1.800 |
| R-factor | 0.2167 |
| Rwork | 0.215 |
| R-free | 0.25240 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.384 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 | 1.860 |
| High resolution limit [Å] | 1.800 | 2.030 | 1.800 |
| Number of reflections | 50087 | ||
| Completeness [%] | 98.2 | 98.4 | 95.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.7 | 293 | 60% MPD, 0.1M Bicine, pH 9.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
