4KN1
Human folate receptor beta (FOLR2) in complex with the antifolate aminopterin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-01 |
Detector | NOIR-1 |
Wavelength(s) | 1.2802 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 97.075, 97.075, 98.955 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.538 - 2.301 |
R-factor | 0.1914 |
Rwork | 0.188 |
R-free | 0.23590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kmz |
RMSD bond length | 0.004 |
RMSD bond angle | 0.845 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.155 | 0.078 | 0.598 |
Number of reflections | 12567 | ||
<I/σ(I)> | 5.8 | ||
Completeness [%] | 98.4 | 98.8 | 97.4 |
Redundancy | 10.6 | 9.3 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M Lithium sulfate, 0.1 M Tris-HCl pH 8.0, 20% (w/v) PEG 3350, Vapor diffusion, sitting drop, temperature 293K |