4KMX
Human folate receptor alpha (FOLR1) at acidic pH, hexagonal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-03-01 |
| Detector | APS-1/SBC-1 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 |
| Unit cell lengths | 99.193, 99.193, 56.869 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.635 - 2.200 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.852 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.960 | 2.200 |
| Rmerge | 0.096 | 0.038 | 0.574 |
| Number of reflections | 15749 | ||
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 96.4 | 92.5 | 90.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1 M Citrate, pH 4.5, 0.1 M LiCl, 17.5 % PEG 8000, Vapor diffusion, sitting drop, temperature 298K |
