4KLZ
Inhibition of Small GTPases by Stabilization of the GDP Complex, a Novel Approach applied to Rit1, a Target for Rheumatoid Arthritis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2007-02-27 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54146 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.278, 37.048, 40.736 |
| Unit cell angles | 90.00, 95.90, 90.00 |
Refinement procedure
| Resolution | 40.500 - 2.300 |
| R-factor | 0.19786 |
| Rwork | 0.185 |
| R-free | 0.31099 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ery |
| RMSD bond length | 0.042 |
| RMSD bond angle | 3.177 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.500 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 5464 |
| <I/σ(I)> | 36.45 |
| Completeness [%] | 98.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 11-14% w/v PEG4000, 5-8% v/v Jeffamine M600, imidazole , VAPOR DIFFUSION, SITTING DROP, temperature 291K |
