4KLT
DNA polymerase beta mismatched product complex with Mn2+, 30 min
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-24 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.082, 78.596, 54.980 |
Unit cell angles | 90.00, 112.43, 90.00 |
Refinement procedure
Resolution | 25.764 - 1.979 |
R-factor | 0.2322 |
Rwork | 0.229 |
R-free | 0.29250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c2m |
RMSD bond length | 0.007 |
RMSD bond angle | 1.208 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 1.979 | 1.979 |
Rmerge | 0.090 | 0.433 |
Number of reflections | 29614 | |
<I/σ(I)> | 15.9 | 2.217 |
Completeness [%] | 97.7 | 89.3 |
Redundancy | 5.1 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, temperature 291K |