4KLS
DNA polymerase beta mismatched reactant complex with Mn2+, 10 min
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-25 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.920, 78.607, 55.034 |
Unit cell angles | 90.00, 112.88, 90.00 |
Refinement procedure
Resolution | 24.128 - 1.978 |
R-factor | 0.2292 |
Rwork | 0.226 |
R-free | 0.28530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c2m |
RMSD bond length | 0.015 |
RMSD bond angle | 1.229 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 1.978 | 1.978 |
Rmerge | 0.078 | 0.418 |
Number of reflections | 30106 | |
<I/σ(I)> | 14.3 | 2.45 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 4.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, temperature 291K |