4KLQ
Observing a DNA polymerase choose right from wrong.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-13 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.822, 78.412, 54.962 |
Unit cell angles | 90.00, 113.24, 90.00 |
Refinement procedure
Resolution | 24.036 - 1.999 |
R-factor | 0.2176 |
Rwork | 0.214 |
R-free | 0.26720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c2m |
RMSD bond length | 0.013 |
RMSD bond angle | 1.225 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 1.999 | 1.999 |
Rmerge | 0.062 | 0.385 |
Number of reflections | 27926 | |
<I/σ(I)> | 15.1 | 2.397 |
Completeness [%] | 96.4 | 78.2 |
Redundancy | 4.4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, temperature 291K |