4KI8
Crystal structure of a GroEL-ADP complex in the relaxed allosteric state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 235.222, 141.655, 156.693 |
| Unit cell angles | 90.00, 113.84, 90.00 |
Refinement procedure
| Resolution | 46.166 - 2.722 |
| R-factor | 0.1662 |
| Rwork | 0.166 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xck |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.955 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.170 | 46.170 | 2.820 |
| High resolution limit [Å] | 2.720 | 5.860 | 2.720 |
| Number of reflections | 120240 | ||
| <I/σ(I)> | 13.98 | ||
| Completeness [%] | 95.6 | 98.9 | 85.3 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 34% MPD (v/v), 0.1M acetic acid, 20mM CaCl2, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
