4KGO
Crystal Structure of double Leucine to Methionine mutant human splunc1 lacking the secretion signal sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-02-20 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 4 |
| Unit cell lengths | 151.827, 151.827, 35.943 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.012 - 3.194 |
| R-factor | 0.2297 |
| Rwork | 0.229 |
| R-free | 0.25570 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.861 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.260 |
| High resolution limit [Å] | 3.194 | 8.680 | 3.194 |
| Rmerge | 0.109 | 0.069 | 0.759 |
| Number of reflections | 14004 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 99.1 | 98.6 | 90.8 |
| Redundancy | 14.5 | 13.9 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 8% PEG 400, .2 M tri-sodium citrate, .1 M Tris-HCl pH 8.5, vapor diffusion, temperature 298K |
