4KGO
Crystal Structure of double Leucine to Methionine mutant human splunc1 lacking the secretion signal sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-02-20 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9794 |
Spacegroup name | P 4 |
Unit cell lengths | 151.827, 151.827, 35.943 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.012 - 3.194 |
R-factor | 0.2297 |
Rwork | 0.229 |
R-free | 0.25570 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.861 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 3.260 |
High resolution limit [Å] | 3.194 | 8.680 | 3.194 |
Rmerge | 0.109 | 0.069 | 0.759 |
Number of reflections | 14004 | ||
<I/σ(I)> | 7.5 | ||
Completeness [%] | 99.1 | 98.6 | 90.8 |
Redundancy | 14.5 | 13.9 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | 8% PEG 400, .2 M tri-sodium citrate, .1 M Tris-HCl pH 8.5, vapor diffusion, temperature 298K |