4KCV
Pyruvate kinase (PYK) from Trypanosoma brucei soaked with 2-oxoglutaric acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 103.250, 108.010, 265.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.630 - 2.180 |
R-factor | 0.15934 |
Rwork | 0.158 |
R-free | 0.19341 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.039 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.630 | 2.300 |
High resolution limit [Å] | 2.180 | 2.180 |
Number of reflections | 77484 | |
Completeness [%] | 100.0 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 15-20% PEG 8,000, 10-20% glycerol, 80 uM F26BP, 800 uM Ponceau S, 50 mM TEA, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |