4KBR

Crystal structure of mouse Ceramide-1-phosphate transfer protein (apo-form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	81.997, 83.876, 268.721
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.507 - 2.547
R-factor	0.2042
Rwork	0.201
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Human CPTP in complex with 2:0 C1P
RMSD bond length	0.009
RMSD bond angle	1.151
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.550	5.490	2.550
Rmerge	0.086	0.032	0.596
Number of reflections	57653
<I/σ(I)>	8.3
Completeness [%]	93.5	99.8	85.4
Redundancy	10	9.8	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M Bis-Tris pH 6.5, 2 M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K