4KB6
Structure of porcine cyclic GMP AMP synthase (CGAS) in complex with DNA, ATP and GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2013-03-14 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97626 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 86.280, 111.890, 117.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.325 - 3.075 |
R-factor | 0.2547 |
Rwork | 0.255 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jlx |
RMSD bond length | 0.004 |
RMSD bond angle | 0.888 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 999.000 | 999.000 | 3.250 |
High resolution limit [Å] | 3.070 | 9.150 | 3.070 |
Number of reflections | 10762 | ||
Completeness [%] | 98.6 | 98.9 | 91.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.05 M Na-cacodylate, 2.5 mM spermine, 60 mM magnesium chloride, 3 % PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |