4KAT
Crystal structure of FDTS from T. maritima mutant (R174K) with FAD and dUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.680, 115.740, 140.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.580 - 2.140 |
| R-factor | 0.18507 |
| Rwork | 0.183 |
| R-free | 0.23260 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1o26 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.716 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.700 | 2.200 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.073 | 0.885 |
| Number of reflections | 49181 | |
| <I/σ(I)> | 17.8 | 2.21 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 50 - 60% PEG200, 0.1Tris.HCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 8.0 |
