4KAQ
Structure of rituximab Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.650, 93.930, 144.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.320 - 2.480 |
| R-factor | 0.1822 |
| Rwork | 0.180 |
| R-free | 0.23030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2osl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.808 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1320)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.320 | 2.540 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.058 | 0.199 |
| Number of reflections | 14891 | |
| <I/σ(I)> | 22.4 | 6.3 |
| Completeness [%] | 99.2 | 92.4 |
| Redundancy | 4.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 22% PEG 3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
