4K96
Structure of Binary Complex of cGAS with Bound dsDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2013-01-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9790 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 181.910, 93.801, 75.509 |
Unit cell angles | 90.00, 97.72, 90.00 |
Refinement procedure
Resolution | 45.066 - 2.084 |
R-factor | 0.2023 |
Rwork | 0.202 |
R-free | 0.22630 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.947 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.084 | 2.084 |
Number of reflections | 74352 | |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 0.1 M MES, 8% MPD, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |