4K74
The UmuC subunit of the E. coli DNA polymerase V shows a unique interaction with the beta-clamp processivity factor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.520, 66.170, 82.730 |
| Unit cell angles | 90.00, 114.99, 90.00 |
Refinement procedure
| Resolution | 70.000 - 2.500 |
| R-factor | 0.23746 |
| Rwork | 0.231 |
| R-free | 0.29470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d1g |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.372 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.070 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.062 | 0.284 |
| Number of reflections | 46830 | |
| <I/σ(I)> | 7.7 | 2.5 |
| Completeness [%] | 93.6 | |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 285 | 200 mM calcium acetate, 200 mM MES pH 6.5, 14% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
