4K6O
X-ray structure uridine phosphorylase from Vibrio cholerae in complex with 6-methyluracil at 1.17 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.826 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.012, 97.100, 93.017 |
| Unit cell angles | 90.00, 119.99, 90.00 |
Refinement procedure
| Resolution | 40.280 - 1.170 |
| R-factor | 0.11 |
| Rwork | 0.108 |
| R-free | 0.12800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ip0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.185 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.560 | 1.200 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.038 | 0.204 |
| Number of reflections | 449315 | |
| <I/σ(I)> | 11.95 | 3.23 |
| Completeness [%] | 90.7 | 55.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 286 | PEG4000, 0.1M TRIS-HCl, 0.2M MgCl2x6H2O, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
