4K6M
Crystal Structure of the full-length Japanese encephalitis virus NS5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979305 |
Spacegroup name | H 3 |
Unit cell lengths | 272.293, 272.293, 177.248 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.219 - 2.600 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2px2 2j7u |
RMSD bond length | 0.008 |
RMSD bond angle | 1.164 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.200 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 148758 | |
Completeness [%] | 98.7 | 97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.085M trisodium citrate pH 5.6, 0.17M potassium/sodium tartrate, 1.7M ammonium sulfate, 15%(v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |