4K50

Rhinovirus 16 polymerase elongation complex (r1_form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Detector	NOIR-1
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	53.750, 113.893, 122.600
Unit cell angles	90.01, 90.49, 90.00

Refinement procedure

Resolution	45.303 - 2.930
R-factor	0.1935
Rwork	0.191
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.187
Data reduction software	d*TREK (9.9.9.1L)
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.070	49.070	3.030
High resolution limit [Å]	2.930	6.310	2.930
Rmerge	0.108	0.059	0.438
Total number of observations		12162	12283
Number of reflections	61473
<I/σ(I)>	5.1	13.1	1.6
Completeness [%]	98.4	97.1	98.3
Redundancy	1.98	1.99	1.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.16 M ammonium sulfate, 0.08 M sodium acetate, pH 4.6, 20% (w/v) PEG 4000, and 20% (v/v) glycerol and directly frozen , VAPOR DIFFUSION, SITTING DROP, temperature 289K