4K4X

Coxsackievirus B3 polymerase elongation complex (r2_form), rna

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Detector	NOIR-1
Spacegroup name	P 1
Unit cell lengths	61.001, 61.013, 195.137
Unit cell angles	90.02, 89.98, 78.36

Refinement procedure

Resolution	44.010 - 2.370
R-factor	0.205
Rwork	0.204
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.100
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.780	2.450
High resolution limit [Å]	2.370	2.370
Rmerge	0.064	0.302
Number of reflections	109647
<I/σ(I)>	6.8	1.5
Completeness [%]	98.0	96.6
Redundancy	1.99	1.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	289	0.17 M sodium acetate, 0.085 M Tris, 25.5% PEG 4000, and 15% glycerol and directly frozen, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K