4K35
The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.042, 118.802, 139.422 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.122 - 2.003 |
R-factor | 0.1436 |
Rwork | 0.143 |
R-free | 0.17680 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.106 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.313 | |
Number of reflections | 106346 | |
<I/σ(I)> | 9.75 | |
Completeness [%] | 99.9 | 98.3 |
Redundancy | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG4000, Tris, MPD, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |