4K31
Crystal structure of apramycin bound to the leishmanial rRNA A-site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9394 |
Spacegroup name | P 31 |
Unit cell lengths | 32.790, 32.790, 107.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.510 - 1.415 |
R-factor | 0.2277 |
Rwork | 0.226 |
R-free | 0.26440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g5k |
RMSD bond length | 0.006 |
RMSD bond angle | 0.976 |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.800 | 1.490 |
High resolution limit [Å] | 1.400 | 1.420 |
Rmerge | 0.048 | 0.137 |
Number of reflections | 23325 | |
<I/σ(I)> | 14.1 | 5.7 |
Completeness [%] | 94.5 | 98 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 50 mM Na cacodylate, 1 mM spermine tetrahydrochloride, 1% 2-methyl-2,4-pentadiol (vs. 40% reservoir), 100-250 mM NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |