4K2P
The Structure of a Quintuple Mutant of the Tiam1 PH-CC-Ex Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-07 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.507, 70.111, 87.753 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 19.703 - 1.980 |
| R-factor | 0.1938 |
| Rwork | 0.192 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a8p |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.703 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.047 | 0.357 |
| Number of reflections | 67495 | |
| <I/σ(I)> | 14.3 | 2.3 |
| Completeness [%] | 99.0 | 97.6 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.2 M lithium sulfate, 0.1 M Tris, 19% PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
