4K2P
The Structure of a Quintuple Mutant of the Tiam1 PH-CC-Ex Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-07 |
Detector | NOIR-1 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.507, 70.111, 87.753 |
Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
Resolution | 19.703 - 1.980 |
R-factor | 0.1938 |
Rwork | 0.192 |
R-free | 0.23590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a8p |
RMSD bond length | 0.006 |
RMSD bond angle | 0.955 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.703 | 2.090 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.047 | 0.357 |
Number of reflections | 67495 | |
<I/σ(I)> | 14.3 | 2.3 |
Completeness [%] | 99.0 | 97.6 |
Redundancy | 2.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.2 M lithium sulfate, 0.1 M Tris, 19% PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |