4K2E
HlyU from Vibrio cholerae N16961
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-07-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.334, 42.188, 88.288 |
| Unit cell angles | 90.00, 93.60, 90.00 |
Refinement procedure
| Resolution | 26.530 - 1.800 |
| R-factor | 0.1919 |
| Rwork | 0.189 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jth |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.303 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.531 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.044 | 0.496 |
| Number of reflections | 36262 | |
| <I/σ(I)> | 10.4 | 1.8 |
| Completeness [%] | 99.0 | 99.86 |
| Redundancy | 2.75 | 2.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 8000, 0.05M potassium phosphate monobasic, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
