4K22
Structure of the C-terminal truncated form of E.Coli C5-hydroxylase UBII involved in ubiquinone (Q8) biosynthesis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-12 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.980110 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 94.730, 124.140, 71.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.980 - 2.000 |
R-factor | 0.1643 |
Rwork | 0.163 |
R-free | 0.19810 |
Structure solution method | SAD then MR |
RMSD bond length | 0.012 |
RMSD bond angle | 1.253 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.980 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.067 |
Number of reflections | 58014 |
<I/σ(I)> | 14.5 |
Completeness [%] | 99.5 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1M Tris pH 8.5, 12% PEG 4000, 0.1M NaCl, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
2 | VAPOR DIFFUSION, HANGING DROP | 9 | 292 | 0.1M BisTris-propane pH 9.0, 14% PEG 4000, 0.1M NaCl, 0.15M MgCl2', VAPOR DIFFUSION, HANGING DROP, temperature 292K |