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4K22

Structure of the C-terminal truncated form of E.Coli C5-hydroxylase UBII involved in ubiquinone (Q8) biosynthesis

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSOLEIL BEAMLINE PROXIMA 1
Synchrotron siteSOLEIL
BeamlinePROXIMA 1
Temperature [K]100
Detector technologyPIXEL
Collection date2012-09-12
DetectorPSI PILATUS 6M
Wavelength(s)0.980110
Spacegroup nameP 21 21 2
Unit cell lengths94.730, 124.140, 71.890
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution46.980 - 2.000
R-factor0.1643
Rwork0.163
R-free0.19810
Structure solution methodSAD then MR
RMSD bond length0.012
RMSD bond angle1.253
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER (2.5.2)
Refinement softwarePHENIX ((phenix.refine: 1.8.2_1309))
Data quality characteristics
 Overall
Low resolution limit [Å]46.980
High resolution limit [Å]2.000
Rmerge0.067
Number of reflections58014
<I/σ(I)>14.5
Completeness [%]99.5
Redundancy5.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8.52920.1M Tris pH 8.5, 12% PEG 4000, 0.1M NaCl, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 292K
2VAPOR DIFFUSION, HANGING DROP92920.1M BisTris-propane pH 9.0, 14% PEG 4000, 0.1M NaCl, 0.15M MgCl2', VAPOR DIFFUSION, HANGING DROP, temperature 292K

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