4K17
Crystal Structure of mouse CARMIL residues 1-668
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9784 |
Spacegroup name | P 1 |
Unit cell lengths | 57.168, 67.986, 212.151 |
Unit cell angles | 92.73, 96.95, 110.15 |
Refinement procedure
Resolution | 49.759 - 2.895 |
Rwork | 0.215 |
R-free | 0.25890 |
Structure solution method | SAD |
RMSD bond length | 0.023 |
RMSD bond angle | 2.308 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.895 | 2.895 |
Rmerge | 0.124 | 0.825 |
Number of reflections | 63752 | |
<I/σ(I)> | 13.8 | 1.8 |
Completeness [%] | 98.0 | 84.4 |
Redundancy | 8 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 293 | 16 % PEG3350, 130 mM lithium sulfate, 0.25 % w/v salicylamide, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |