4K0W
X-ray crystal structure of OXA-23 A220 duplication clinical variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-07-28 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 44.116, 46.662, 137.018 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 137.000 - 1.200 |
| R-factor | 0.18335 |
| Rwork | 0.182 |
| R-free | 0.20212 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 137.000 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Number of reflections | 103356 | |
| Completeness [%] | 94.7 | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 296 | 0.2M sodium chloride, 0.1M phosphate, 0.1M citrate 20% w/v PEG 8000 , pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
