4K0W
X-ray crystal structure of OXA-23 A220 duplication clinical variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-07-28 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 44.116, 46.662, 137.018 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 137.000 - 1.200 |
R-factor | 0.18335 |
Rwork | 0.182 |
R-free | 0.20212 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 137.000 | 1.140 |
High resolution limit [Å] | 1.100 | 1.100 |
Number of reflections | 103356 | |
Completeness [%] | 94.7 | 94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 296 | 0.2M sodium chloride, 0.1M phosphate, 0.1M citrate 20% w/v PEG 8000 , pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 296K |