4JZV
Crystal structure of the Bacillus subtilis pyrophosphohydrolase BsRppH bound to a non-hydrolysable triphosphorylated dinucleotide RNA (pcp-pGpG) - second guanosine residue in guanosine binding pocket
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-27 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 62.158, 138.025, 35.684 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.340 - 2.200 |
R-factor | 0.221 |
Rwork | 0.220 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | STRUCTURE OF BSRPPH E68A |
RMSD bond length | 0.007 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.010 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.057 | 0.640 |
Number of reflections | 16071 | |
<I/σ(I)> | 18.1 | 2.3 |
Completeness [%] | 98.6 | 95.1 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 293 | 100 mM Hepes pH 7.5, 25% PEG 1000, VAPOR DIFFUSION, temperature 293K |