4JZQ
Crystal structure of human CLIC1 C24D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.381, 69.691, 83.420 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 27.807 - 1.350 |
| R-factor | 0.1527 |
| Rwork | 0.151 |
| R-free | 0.18210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k0m |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.527 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.480 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.053 | 0.265 |
| Number of reflections | 99542 | |
| <I/σ(I)> | 12.1 | 3.3 |
| Completeness [%] | 93.6 | 67.6 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 2% Tacsimate, 0.1M Bis-Tris, 20% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
