4JXT

CID of human RPRD1A in complex with a phosphorylated peptide from RPB1-CTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Detector technology	IMAGE PLATE
Collection date	2011-09-27
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	I 4
Unit cell lengths	93.264, 93.264, 36.027
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.510 - 1.900
R-factor	0.187
Rwork	0.184
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hfg
RMSD bond length	0.014
RMSD bond angle	1.469
Data reduction software	XDS
Data scaling software	SCALA (CCP4_3.3.9)
Phasing software	PHASER
Refinement software	REFMAC (5.7.0027)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge	0.119	0.030	0.930
Total number of observations		2939	12320
Number of reflections	12196
<I/σ(I)>	14.7596
Completeness [%]	98.3	99.11	96.34
Redundancy	7.3	7	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	20% PEG-4000, 10% isopropanol, 0.1M HEPES., pH 7.5, vapor diffusion, temperature 291K
1	VAPOR DIFFUSION	7.5	291	20% PEG-4000, 10% isopropanol, 0.1M HEPES., pH 7.5, vapor diffusion, temperature 291K